Journal: Nature Communications

Article Title: Neoantigen enriched biomimetic nanovaccine for personalized cancer immunotherapy

doi: 10.1038/s41467-025-59977-8

Figure Lengend Snippet: a MHC-I surface expression on B16-OVA cells post 1788 treatments. b MHC-I surface expression on indicated murine cell lines and the cell viabilities relative to control by different treatments. SKCM Skin Cutaneous Melanoma, COAD Colorectal Adenocarcinoma, PDAC Pancreatic Ductal Adenocarcinoma, TNBC Triple-Negative Breast Cancer, LUAD Lung Adenocarcinoma. c Representative FACS histograms and quantification of MHC-I expression on B16-OVA cells with or without IFN-γ treatment (n = 3 independent experiments). d OVA presentation on B16-OVA cells and cell viabilities by different treatments. e Representative FACS histograms and quantification showing OVA-presentation on IFN-γ treated and control B16-OVA cells. Dashed lines, isotype; solid lines, anti-Kb-SIINFEKEL. (n = 3 independent experiments). f HLA-I surface levels on indicated human cell lines with or without IFN-γ treatment. AML Acute Myeloid Leukemia, BRCA Breast Cancer, CHOL Cholangiocarcinoma, DCIS Ductal Carcinoma in Situ, GBM Glioblastoma Multiforme, LIHC Liver Hepatocellular Carcinoma, LUSC Lung Squamous Cell Carcinoma, NBL Neuroblastoma, OPSCC Oropharyngeal Squamous Cell Carcinoma, OV Ovarian Cancer, RCC Renal Cell Carcinoma, SARC Sarcoma, STAD Stomach Adenocarcinoma, DLBC Diffuse Large B-Cell Lymphoma, OS Osteosarcoma. g Western blot showing Na +/ K + -ATPase and MHC-I in the cell membranes and cell lysates of B16-OVA cells with or without IFN-γ treatment. h OVA-presentation on AECMs or CMs of B16-OVA cells as determined by flow cytometry. OT-I T-cells were incubated with 2 μg/mL B16-OVA derived CM or AECM for 48 days, T-cell proliferation rate ( i ) and CD69 expression ( j ) in CD8 + T-cells were shown (n = 3 independent experiments). OT-I T-cells were incubated with B16-OVA derived CM or AECM for 3 days, T-cell proliferation rate ( k ) and CD69 expression ( l ) in CD8 + T-cells were shown (n = 3 independent experiments). In ( c , e , h –l ), representative data from three independent experiments are presented as means ± s.e.m. Statistical significance was calculated by ordinary one-way ANOVA ( i , j ) and Student’s two-sided unpaired t -test ( c right, e right, h , k , l ). Source data are provided as a file.

Article Snippet: Anti-Sodium Potassium ATPase Rabbit Monoclonal Antibody (AF1864), anti- Histone H3 Mouse Monoclonal Antibody (AF0009), anti–β-Tubulin rabbit monoclonal antibody (AF1216), HRP–labelled goat anti-mouse IgG (H + L) (A0216), and HRP–labelled goat anti-rabbit IgG (H + L) (A0208) were obtained from Beyotime.

Techniques: Expressing, Control, In Situ, Western Blot, Flow Cytometry, Incubation, Derivative Assay

Journal: eLife

Article Title: Identification of nonsense-mediated decay inhibitors that alter the tumor immune landscape

doi: 10.7554/eLife.95952

Figure Lengend Snippet: ( A ) Western blot showing SPTAN1 expression after treatment with dimethyl sulfoxide (DMSO), 5 µM LY3023414, or 1 µM SMG1 inhibitor SMG1i-11 (lanes 1, 2, and 3, respectively, for each antibody). The arrow indicates the expected size of the mutant NMD-targeted protein. Antibody ab75755 (Abcam) binds C-terminal to the out-of-frame indel and does not show mutant protein as expected. Antibody A301-249 (Bethyl) is polyclonal and also did not bind mutant protein. Antibody ab11755 (Abcam) is located N-terminal to the indel and does display mutant protein expression. ( B ) Western blot showing EXOC1 expression after treatment with DMSO, 5 µM LY3023414, or 1 µM SMG1i-11 (lanes 1, 2, and 3, respectively). The arrow indicates the expected size of the mutant (NMD-targeted) protein.

Article Snippet: Antibody , Rabbit anti Sodium Potassium Pump (Na-K) polyclonal , Cell Signaling Technology , 3010S, RRID: AB_2060983 , Used for western blot at 1:500.

Techniques: Western Blot, Expressing, Mutagenesis

Journal: eLife

Article Title: Identification of nonsense-mediated decay inhibitors that alter the tumor immune landscape

doi: 10.7554/eLife.95952

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit anti Sodium Potassium Pump (Na-K) polyclonal , Cell Signaling Technology , 3010S, RRID: AB_2060983 , Used for western blot at 1:500.

Techniques: Western Blot, Flow Cytometry, Sequencing, Expressing, Plasmid Preparation, Luciferase, Transfection, Formulation, Saline, Gene Expression, Real-time Polymerase Chain Reaction, Protease Inhibitor, Recombinant, Lysis, Extraction, Drug discovery, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Knock-Out, CRISPR, Mutagenesis, Software